• There should be no oxygen in the fermenter (production tank-digester).

• Animal wastes that have received antibiotics should not be inserted into the production tank.

• Organic waste with detergents should not be inserted into the production tank,

• A sufficient amount of nitrogen should be present for the formation and growth of new bacteria in the environment.

• Acidity in the production tank should be pH =  7.0 – 7.6.

• Organic acid concentration should be around 1,500 mg / liter.

• Temperature in the digester should be constant at between 35°C to 56°C.

• Environment should be dark. No light should enter the digester.

• Optimum water content in the digester is 90%. In no case should this be less than 50%.

• There should be sufficient amount of organic material to feed methane bacteria.